Thermodynamic characterization of calcium-milk protein interaction by isothermal titration calorimetry
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چکیده
منابع مشابه
Characterization of membrane protein interactions by isothermal titration calorimetry.
Understanding the structure, folding, and interaction of membrane proteins requires experimental tools to quantify the association of transmembrane (TM) helices. Here, we introduce isothermal titration calorimetry (ITC) to measure integrin αIIbβ3 TM complex affinity, to study the consequences of helix-helix preorientation in lipid bilayers, and to examine protein-induced lipid reorganization. P...
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Of all the techniques that are currently available to measure binding, isothermal titration calorimetry is the only one capable of measuring not only the magnitude of the binding affinity but also the magnitude of the two thermodynamic terms that define the binding affinity: the enthalpy (AH) and entropy (AS) changes. Recent advances in instrumentation have facilitated the development of experi...
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ژورنال
عنوان ژورنال: Dairy Science and Technology
سال: 2009
ISSN: 1958-5586,1958-5594
DOI: 10.1051/dst/2009006